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Taking productivity to the next level A true evolution in flow cytometry is empowered by BD FACSuite™ software. It enables BD analyzers to provide standardization, consistency and flexibility while generating the highest quality reproducible data possible with the technology available today. For Research Use Only. 642224 6/07 Updated to include BD FACSDiva software, version 6.0, added configuration and performance tracking module, operational improvements, and increased cytometer-specific improvements. 23-11232-00 Rev. 01 4/2012 Updated for BD FACSDiva 7.0 software and BD LSRFortessa flow cytometer.

BD FACSDiva™ software is a collection of rich tools for flow cytometer and application setup, data acquisition, and data analysis that help streamline flow cytometry workflows for today’s busy laboratory.

BD FACSDiva software provides new features to help users integrate flow systems into new application areas, including index sorting for stem cell and single-cell applications, as well as automation protocols for high-throughput and robotic laboratories.

BD FACSDiva™ CS&T Bead Lot Files (BD FACSDiva v7.0 and later)

Each lot of BD FACSDiva CS&T research beads has an associated bead lot file that needs to be downloaded from this website and then imported into BD FACSDiva software v7.0 or later.

Download Instructions

To download and install the appropriate bead lot file, please follow these instructions. (You may wish to print them.)

BD FACSDiva™ CS&T Bead Lot Files (BD FACSDiva v7.0 and later)

to set up the software for your use. This guide shows a workflow that uses application settings. BD FACSDiva Software Quick Reference Guide for the BD LSR II. Compensation Controls in the BD FACSDiva Software Reference Manual. • Default templates are now provided for certain instrument functions. If you are the first user of the day: Boot up the computer and log on to Windows. Wait 10 seconds, then Open the BD FACSDiva software and log on. Allow the.

Product name, part number, and serial number; software version and computer system specifications Error messages, if any Details of recent cytometer performance BD Biosciences might also request the console. To move objects between worksheets, use the copy and paste functions; objects cannot be dragged.

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For example, the Inspector can be used to change plot attributes like the background color, title, axes labels, and scale, or to enter the name of an experiment, specimen, or tube. The starting value the lowest level is half the value entered. When recording is complete and acquisition is stopped, statistics are calculated on the faczdiva number of recorded events. Whether viewed on one monitor or two, workspace windows can be resized and repositioned for the most efficient operator workflow.

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Copy and paste wells to blank positions in the Plate window. See Adding New Elements to the Browser on page Additional preferences might be available for your fxcsdiva type; refer to your instrument manual for a description.

A maximum of 50 global worksheets can be set up for each experiment, and each global worksheet can contain up to 10 pages. After you move or resize a snap-to gate, the gate no longer adjusts automatically. The plot expands to twice its size. This material briefly explains how information is stored in Windows More information.

NOTE If a workstation does not have a D drive, then the default locations for output files will be created on the C drive. To start the software: If you set the default background to black, select the checkbox mxnual print plots with a white background; white gates and populations are then automatically printed in black. Comments can be viewed when you are importing a panel template. Video display images copyright More information.

Instrument Name During software installation, each instrument is assigned a name and serial number. This section describes how to subset populations using the population hierarchy. Only one object can be copied and pasted at a time.

BD FACSCANTO II QUICK REFERENCE MANUAL Pdf Download.

If a worksheet with the name of siftware specimen and tube already exists, the copied elements are pasted into the existing worksheet. As you change fluorochromes, filters, or mirrors, you can softsare between mahual configurations that match your instrument optics.

Locate and read topics specific to the operation you are performing. For example, experiment templates can be assigned to the New Experiment button, panel templates can be assigned to the New Specimen button, and analysis templates can be assigned to the New Tube or New Global Worksheet button. This section describes how to export experiments and delete them after export; deleting experiments is described on page Select this checkbox to always update the cytometer settings to the latest performance check.

Close the region by double-clicking or by clicking on the first vertex. No part of this publication may be reproduced, transmitted, transcribed, stored. The image is stored in memory until it is pasted into another application.

Start display at page:. All tubes must be recorded with consistent PMT voltages.

BD FACSCanto II Quick Reference Manual

Statistics are displayed in statistics views and the values are updated in accordance with the Events to Display setting. Edit this number by selecting one or more fields and then entering a new number.

To move a window, drag the title bar to a new position on the screen. Cytometer settings can be applied at the experiment, specimen, or tube level. The computer is left on at all times. Otherwise, generic value is applied. Provides an interface for setting up experiments. Laser Controls Lasers are instrument-specific, thus laser controls for your instrument might be different from those shown in this section. The chosen worksheet is displayed automatically when you select a tube below this specimen.

The following tutorials describe how to add an administrator password and create a new user, and show how workspace setup, user preferences, and the Browser view differ from one user to another. A summary of menus is provided in Shortcut Menus on page Using Gating Features Acquisition Setup Use Acquisition Setup fields to control the number of events to record and display.

BD Biosciences welcomes customer input on corrections and suggestions for improvement. Global worksheets are displayed in this folder in the order they were created.

BD FACSDiva Software Reference Manual

Interval Gate button—allows a range of events to be selected on a dot, contour, or histogram plot. If needed, start your cytometer and log into the software before proceeding.

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Click the intersection of the quadrant markers.

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